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Item #:
253700399
SDS Buffer, 10% Sodium Dodecyl Sulfate Solution for Lab, 500mL2
85% of respondents would recommend this to a friend
Item #:
253700399
XCD 119
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What Stands Out
High Purity SDS
This SDS buffer features 10% high-purity sodium dodecyl sulfate, ensuring reliable and reproducible results for lab applications in protein analysis and electrophoresis.
Large Volume
With a generous 500mL quantity, this solution meets the demands of intensive laboratory work, minimizing the need for frequent replenishments and ensuring continuous workflow.
Versatile Applications
Perfectly suited for various experiments, this SDS buffer is ideal for applications including DNA extraction, protein denaturation, and SDS-PAGE, making it an essential tool for researchers.
Product Details
| Brand Name | CryoKing |
| Item Highlight | 10% SDS solution for SDS-PAGE, protein electrophoresis, plasmid extraction and molecular biology workflows |
| Unit Count | 2 Count |
| Manufacturer | BIOLOGIX |
| Included Components | 10% SDS Buffer |
| Part Number | SDS-500-2 |
| Brand | CryoKing |
| Item Highlight | 10% SDS solution for SDS-PAGE, protein electrophoresis, plasmid extraction and molecular biology workflows See more |
| Item Weight | 1.1 lbs (500 grams) |
Product Description
SDS Buffer, 10% Sodium Dodecyl Sulfate Solution for Lab, 500mL2
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Customer Questions & Answers
-
Question:
What is Lab SDS Buffer and what is it used for?
Answer: Lab SDS Buffer, specifically 10% Sodium Dodecyl Sulfate, is a powerful ionic detergent commonly used in molecular biology. Its primary role is to denature proteins, providing a consistent negative charge that allows proteins to be separated by size during gel electrophoresis. This is essential for applications such as protein analysis, sample preparation, and Western blotting. -
Question:
How should I store Lab SDS Buffer?
Answer: Lab SDS Buffer should be stored at room temperature away from direct sunlight, in a tightly sealed container to prevent contamination. Proper storage is crucial as it ensures the buffer maintains its effectiveness for protein denaturation and other biochemical assays, ultimately leading to reliable experimental results. -
Question:
Is Lab SDS Buffer safe to handle?
Answer: While Lab SDS Buffer is commonly used in laboratories, it is important to handle it with care. As a detergent, SDS can be irritating to the skin and eyes. Always use personal protective equipment, such as gloves and goggles, when working with it to ensure safety and minimize exposure. -
Question:
Can Lab SDS Buffer be used for DNA analysis?
Answer: Lab SDS Buffer is primarily designed for protein applications but can also play a role in DNA extraction processes. Its ability to lyse cells and denature proteins facilitates the release of nucleic acids. However, for specific DNA procedures, other buffers may be more effective, so it's wise to refer to established protocols. -
Question:
What volume of buffer is included in each case?
Answer: Each case of Lab SDS Buffer contains two bottles, with each bottle containing 500ml of the buffer solution. This volume is suitable for multiple laboratory applications, making it an efficient choice for researchers performing extensive experiments that require consistent SDS preparation. -
Question:
What are potential alternatives to Lab SDS Buffer?
Answer: Alternatives to Lab SDS Buffer include other detergents like Triton X-100 and Tween 20, which may be more suitable for specific applications depending on the protein or sample type. When choosing a detergent, consider factors like the nature of the proteins being analyzed and the specific requirements of your experimental setup. -
Question:
How do I prepare for an experiment using Lab SDS Buffer?
Answer: Before using Lab SDS Buffer, ensure that you have all necessary equipment such as electrophoresis apparatus, gels, and other reagents prepared. It's paramount to understand your experimental design and the role that SDS plays in protein separation, ensuring that every step is accounted for to achieve optimal results. -
Question:
Can Lab SDS Buffer be reused after an experiment?
Answer: Generally, it is not recommended to reuse Lab SDS Buffer after an experiment. Reusing buffers can introduce contamination and alter the ionic strength or pH, impacting the reproducibility and reliability of your results. It's best to prepare fresh buffers for each experiment to ensure consistency. -
Question:
Does Lab SDS Buffer contain any preservatives?
Answer: Lab SDS Buffer does not typically contain preservatives, as these can interfere with protein analysis. This purity allows for consistent protein denaturation and analysis; however, it necessitates careful handling to avoid contamination from external sources. -
Question:
Where can I buy Lab SDS Buffer in Antigua and Barbuda?
Answer: You can buy Lab SDS Buffer, 10% Sodium Dodecyl Sulfate Buffers, 500ml/Bottle, 2 Bottles/Case from Ubuy. Ubuy offers a convenient online shopping experience, providing a wide selection of laboratory chemicals and supplies tailored to your specific research needs.
CryoKing Buffers Editorial Review
SDS Buffer, 10% Sodium Dodecyl Sulfate Solution for Lab, 500mL2 is an essential tool for various molecular biology workflows, including SDS-PAGE, protein electrophoresis, and plasmid extraction. Manufactured by BIOLOGIX under the CryoKing brand, this 10% SDS solution is reliable for achieving optimal results in labs. With a weight of 1.1 lbs for the 500 grams of solution, it is designed for easy handling and use in research settings. Users have praised its efficiency in protein separation during gel electrophoresis, making it a staple in many laboratory setups.
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Pros
- High-quality for protein electrophoresis
- Effective in plasmid extraction
- Reliable for SDS-PAGE applications
- Easy to handle with 500mL volume
- Trusted brand in molecular biology
Cons
- Requires careful handling and storage conditions
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Features & Benefits
- Advanced technology for biochemical applications.
- Reagent with consistent quality for precise results.
- Long shelf life of at least 3 years at room temperature.
- Low endotoxin levels for enhanced safety.
- Rigorously tested for batch consistency.
- Ideal for various biochemical uses.
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